Toehold‐initiated Rolling Circle Amplification for Visualizing Individual MicroRNAs In Situ in Single Cells

The ability to quantitate and visualize microRNAs (miRNAs) in situ in single cells would greatly facilitate the elucidation of miRNA‐mediated regulatory circuits and their disease associations. A toehold‐initiated strand‐displacement process was used to initiate rolling circle amplification of specific miRNAs, an approach that achieves both stringent recognition and in situ amplification of the target miRNA. This assay, termed toehold‐initiated rolling circle amplification (TIRCA), can be utilized to identify miRNAs at physiological temperature with high specificity and to visualize individual miRNAs in situ in single cells within 3 h. TIRCA is a competitive candidate technique for in situ miRNA imaging and may help us to understand the role of miRNAs in cellular processes and human diseases in more detail.     

Ni-Mo/Al_2O_3催化剂上硫转移反应的研究

制备了以γ-Al2O3为载体的Ni-Mo催化剂,并用于FCC汽油的加氢硫转移反应。对Ni-Mo/Al2O3催化剂上的硫转移反应机理进行了研究,考察了不同反应条件对硫转移反应的影响。实验结果表明,硫醇与烯烃的反应在催化剂表面的加氢活性位上进行,小分子的硫醇发生加氢脱硫,生成吸附态H2S,吸附态的H2S与烯烃反应,生成大分子硫醇和硫醚;另外小分子硫醇还可直接与烯烃发生反应,生成硫醚。提高反应温度、压力、氢油比或降低空速,均可提高催化剂的硫转移反应活性,轻质硫转化率得到明显提高,且单烯选择性还能维持在98%左右。     

Structure,Antibacterial Activity and Theoretical Study of the Forming Mechanism of Compound [(5-Methyl-3-oxo-2-phenyl-1H-pyrazol-4-yl)-thiophen-2-yl-methylene] Thiosemicarbazide

A new thiosemicarbazone compound derived from 1-phenyl-3-methyl-4-(2-thenoyl) pyrazolone-5(HPMTP) and thiosemicarbazide has been synthesized and characterized by IR, HNMR, MS, elemental analysis, UV and single-crystal X-ray diffraction. The compound crystallizes in monoclinic system, Cc space group with a = 7.5925(8), b = 20.263(2), c = 11.4669(13) ,  = 107.825(8)°, V = 1679.5(3) 3, Z = 4, R = 0.0316 and wR = 0.0687. The results of antibacterial activity test against Escherichia coli and Bacillus subtilis indicate that the compound possesses the same antibacterial activity as the contrast(Norfloxacin). Theoretical study of the forming mechanism to the title compound at the RHF/6-31G(d) level shows that there are two steps. The distal amino group of thiosemicarbazide is added to the 4-carbonyl group of HPMTP which forms TM. Then a dehydration reaction occurs in TM and generates a stable product PC.     

Surface effect on band structure of magneto-elastic phononic crystal nanoplates subject to magnetic and stress loadings

This paper presents a theoretical model for the size-dependent band structure of magneto-elastic phononic crystal(PC) nanoplates according to the Kirchhoff plate theory and Gurtin-Murdoch theory, in which the surface effect and magneto-elastic coupling are considered. By introducing the nonlinear coupling constitutive relation of magnetostrictive materials, Terfenol-D/epoxy PC nanoplates are carried out as an example to investigate the dependence of the band structure on the surface effect, magn...     

ITER校正场过渡馈线冷质支撑的设计与传热分析

对过渡馈线的冷质支撑在真空与低温环境下的绝热性能进行研究,以增大热阻抑制热量传递为目的,在保证强度的基础上,同时考虑加工与装配的方便,确定了一种支撑结构.并利用有限元方法对该结构进行了稳态传热分析,得出了支撑的温度分布和冷量损耗大小.研究表明该支撑在满足强度的要求下,不仅结构简单、装配方便而且绝热性能良好,能很好地降低...     

高度均一ZnO纳米梭的水热法制备及其性能

以六亚甲基四胺和硝酸锌为原料,采用水热法在90℃条件下反应24h,于Si衬底上制备出一维ZnO纳米梭材料.采用场发射扫描电镜、X射线衍射仪和高分辨透射电镜等方法对样品形貌和晶体结构进行了表征.结果表明,所得ZnO纳米梭形貌一致、尺寸均匀,为六方纤锌矿型结构,具有良好的结晶性能.对ZnO纳米梭的光致发光性能的研究表明,该...     

基于LabVIEW的激光发散角测试软件开发

根据可变光阑法测量发散角的原理,利用具有强大数值计算与串口通信能力的LabVIEW语言搭建发散角测试软件平台.平台测试结果表明,测试软件具有较好的显示功能与可操作性,同时具有参数设置灵活、可二次开发等优点.在总程序设计的基础上,分别介绍了数据采集、数据处理及数据存储的三个程序模块,最后提出用LabVIEW的循环、嵌套等...     

基于CST的偶极子天线虚拟实验

基于电磁仿真软件CST,设计了偶极子天线虚拟实验,建立了天线仿真模型,给出了仿真结果,不仅解决了实际天线实验设施造价高昂,操作复杂的问题,还可以直观给出天线电流分布、空间电场和磁场分布、辐射方向性图等,为高校电磁场类的物理实验提供一个新的解决方案。     

微型风力发电演示仪

设计制作了一种基于风光互补原理的微型风力发电演示仪.该仪器由风力发电机、光伏太阳能电池板、控制器、蓄电池、负载等各部分组成.整个系统以控制器为核心,由风力发电机、光伏太阳能电池板把风能和光能转变为电能并储存于蓄电池中供负载使用.该仪器作为演示实验仪不仅小型、直观、模块化和操作简便,而且演示内容突显物理与工程相结合的知识...     

高等数学多媒体课堂教学浅议

从使用多媒体讲授高等数学的经验出发,分析高等数学教学中应用多媒体教学手段的优势,同时给出在多媒体教学条件下进一步提高教学效果的几点建议,指出教师必须用现代化的教学思想为指导,综合运用各种教学手段提高高等数学教学效果,适应现代化教学的发展.